Genome-wide identification of essential genes in the invasive Streptococcus anginosus strain.

ABSTRACT: Streptococcus anginosus, part of the Streptococcus anginosus group (SAG), is a human commensal increasingly recognized as an opportunistic pathogen responsible for abscesses formation and infections, also invasive ones. Despite its growing clinical importance, the genetic determinants of its pathogenicity remain poorly understood. This study aimed to identify essential genes in S. anginosus 980/01, a bloodstream isolate, under nutrient-rich laboratory conditions using a transposon mutagenesis combined with Transposon-Directed Insertion Site Sequencing (TraDIS). A mutant library was generated using the ISS1 transposon delivered via the thermosensitive plasmid pGh9:ISS1. Following transposition, insertions were mapped using Illumina sequencing and subsequently analyzed. Essential genes were

SUBMITTER: Kurylek A 

PROVIDER: S-EPMC12464299 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature