Involvement of RNase J in CRISPR RNA maturation in the cyanobacterium <i>Synechocystis</i> sp. PCC 6803.

ABSTRACT: Many bacteria and archaea use CRISPR-Cas systems, which provide RNA-based, adaptive, and inheritable immune defenses against invading viruses and other foreign genetic elements. The proper processing of CRISPR guide RNAs (crRNAs) is a crucial step in the maturation of the defense complexes and is frequently performed by specialized ribonucleases encoded by cas genes. However, some systems employ enzymes associated with degradosome or housekeeping functions, such as RNase III or the endoribonuclease RNase E. Here, the endo- and 5´-exoribonuclease RNase J was identified as an additional enzyme involved in crRNA maturation, acting jointly with RNase E in the crRNA maturation of a type III-Bv CRISPR-Cas system, and possibly together with a further RNase in the cyanobacterium Synechoc

SUBMITTER: Bilger R 

PROVIDER: S-EPMC12464536 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature