Genomic characterization, origin, and local transmission of Oropouche virus in Bolivia in 2024.

ABSTRACT:

Background

The Oropouche virus (OROV) is an arthropod-borne virus that causes an acute febrile illness, like other arboviral diseases. In 2024, Oropouche cases sharply increased in several countries of the Americas, including Bolivia. Here, we investigate the origin and spread of OROV in the Bolivian Amazon region.

Methods

Full-length OROV genomes from 34 positive samples collected in the three affected Bolivian departments during the 2024 outbreak were sequenced using an amplicon-based approach. Maximum Likelihood (ML) phylogenetic analyses of separate viral segments were conducted to identify the responsible viral lineage. Bayesian phylogeographic analysis of concatenated viral segments was used to reconstruct the viral spatiotemporal dispersion pattern within the country.

SUBMITTER: Chuquimia Valdez JA 

PROVIDER: S-EPMC12464684 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature