Optical Control of Membrane Viscosity Modulates ER-to-Golgi Trafficking.

ABSTRACT: The lipid composition of cellular membranes is highly dynamic and undergoes continuous remodeling, affecting the biophysical properties critical to biological function. Here, we introduce an optical approach to manipulate membrane viscosity based on an exogenous synthetic fatty acid with an azobenzene photoswitch, termed FAAzo4. Cells rapidly incorporate FAAzo4 into phosphatidylcholine and phosphatidylethanolamine in a concentration- and cell type-dependent manner. This generates photoswitchable PC and PE analogs, which are predominantly located in the endoplasmic reticulum. Irradiation causes a rapid photoisomerization that decreases membrane viscosity with high spatiotemporal precision. We use the resulting "PhotoCells" to study the impact of membrane viscosity on ER-to-Gol

SUBMITTER: Jimenez-Rojo N 

PROVIDER: S-EPMC12464753 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature