Cell-Membrane-Anchored Synthetic Dynamic DNA Circuits for Signaling Transient Cell Migration.

ABSTRACT: A DNA reaction circuit consisting of components E/Q₁, E₁/T₁, F/Q₁, and F₁/T₁ in which each includes the antimesenchymal epithelial transition (Met) receptor aptamer sequence is anchored within MCF-7 cells to emulate the natural signaling network on the live cell membrane. Subjecting the membrane-integrated circuit to an auxiliary fuel strand, in the presence of a nicking enzyme, results in the dynamic reconfiguration of the circuit into a constitutional dynamic network, CDN, in which the pre-engineered duplex interactions between the constituents lead to allosterically stabilized Met-dimer complexes. The concomitant nickase-induced separation of the CDN leads to the parent reaction circuit, and to the transient formation and

SUBMITTER: Lin N 

PROVIDER: S-EPMC12464974 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature