Bacteriophage T4 propagation in E.coli exposed to severe substrate limitation.

ABSTRACT: Understanding bacteriophage propagation on bacteria in different physiological conditions is imperative for predicting phage therapy efficacy on various bacterial infections, especially chronic ones. We investigated phage T4 propagation on bacteria E.coli grown in a chemostat at very low dilution rates extending down to 0.027 h^-1 and bacteria exposed to nutrient deprivation. An increase in adsorption constant and latent period with dilution rate D decrease and burst size being proportional to dilution rate (D) was confirmed, consistent with previously published results, extending validity of previous findings. Additional bacterial exposure to starvation, either through nutrient cessation or transferring bacteria into SM buffer, sustained phage propagation during first hours of s

SUBMITTER: Lisac A 

PROVIDER: S-EPMC12465135 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature