Integrative analysis of mRNA, lncRNA, circRNA, and miRNA to investigate the anti-fibrotic activity of silibinin in TGF-β2-treated human trabecular meshwork cells.

ABSTRACT:

Background

TGF-β-induced fibrogenic changes in human trabecular meshwork (HTM) cells contribute to intraocular pressure elevation in glaucoma. Silibinin, a natural polyphenolic flavonoid from milk thistle seeds, has been well investigated for its function to inhibit fibrosis. The transcriptomic changes in noncoding RNAs contributing to the pro-fibrotic effect of TGF-β2 and the anti-fibrotic activity of silibinin in HTM cells remain unknown.

Methods

RNA sequencing was conducted to characterize the transcriptomic profiles (mRNA, lncRNA, circRNA, and miRNA) in TGF-β2-stimulated HTM cells, with or without silibinin treatment. Pathway enrichment analysis of the differentially expressed RNAs was conducted. Competing endogenous RNA networks were constructed.

Results

TGF-β2 u

SUBMITTER: Liu J 

PROVIDER: S-EPMC12465171 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature