Automated environmental metagenomics using Oxford nanopore sequencing.

ABSTRACT:

Background

Long-read sequencing has revolutionised metagenomics through improved metagenome assembly, taxonomic classification and functional characterisation. Automation can enhance the throughput, reproducibility, and accuracy of library preparation. However, the validation of automated library preparation protocols remains undetermined for metagenomic workflows, which are particularly sensitive to methodological perturbation. Here, we compare long-read metagenomic sequencing of environmental samples through parallel manual and automated protocols.

Results

Although automated library preparation led to minor reduction in read and contig lengths, taxonomic classification rate and alpha diversity was slightly higher than manual libraries, including the detection of more rare

SUBMITTER: Child HT 

PROVIDER: S-EPMC12465296 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature