Systemic IgG responses to glycosylated mucinase YghJ after experimental enterotoxigenic Escherichia coli infection.

ABSTRACT:

Background

The availability of a broadly protective vaccine against pathogenic Escherichia coli could help to reduce morbidity and mortality from severe gastrointestinal and systemic infections. E. coli vaccine development efforts often target protein virulence factors that natively are extensively glycosylated, but this glycosylation is absent from recombinantly produced vaccine antigens. Human IgA responses to the conserved virulence factor YghJ have recently been shown to frequently target glycosylated epitopes. Here we evaluated to what extent anti-YghJ IgG responses also target glycosylated epitopes, longevity of these responses, and to what extent the responses correlated with the IgA responses.

Methods

Multiplex bead flow cytometric immunoassays were used to evaluate

SUBMITTER: Riaz S 

PROVIDER: S-EPMC12465375 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature