Evaluation of a Chemically Defined, Long-Term Extender for Liquid Storage of Stallion Semen.

ABSTRACT: Efficient use of stallion semen in liquid state is limited by its relatively short shelf-life. A chemically defined extender (Beyond) is now available for long-term liquid semen preservation. The objectives of the present study were to compare Beyond with milk extenders for the preservation of semen at two temperatures, and to evaluate fertility of semen cooled for 4-8 days before artificial insemination. Semen was processed using different extenders: milk, cholesterol (BotuSemen Special); milk-based (INRA 96); and Beyond. Sperm motility, membrane and acrosome integrity, and chromatin structure were evaluated in semen stored at 17°C for 7 days or at 5°C for 14 days. Sperm motility decreased in the first few days of storage regardless of extender or storage temperature. Sperm motility conti

SUBMITTER: Brito LFC 

PROVIDER: S-EPMC12465434 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature