Treg cell-derived exosomal miR-21 promotes osteogenic differentiation of periodontal ligament stem cells.

ABSTRACT:

Background

Regulatory T (Treg) cells are essential for maintaining immune tolerance and have been implicated in tissue regeneration; however, their role in periodontal regeneration remains poorly understood. This study aimed to elucidate the effect of Treg cell-derived exosomes (Treg-Exos), particularly those carrying microRNA-21 (miR-21), on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) and periodontal tissue regeneration.

Methods

After co-culturing Treg cells or Treg-Exos with PDLSCs respectively in vitro, the osteogenic differentiation of treated PDLSCs was assessed through Alkaline phosphatase (ALP) activity, Alizarin Red staining, and the expression of osteogenic markers runt-related transcription factor 2 (Runx-2) and Osterix. The functiona

SUBMITTER: Xia Y 

PROVIDER: S-EPMC12465503 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature