Comparison of the microbiome of sputum from HIV/AIDS patients using PacBio 16S rRNA sequencing and ddPCR.

ABSTRACT:

Background

Pulmonary infections are prevalent opportunistic complications among HIV/AIDS patients, necessitating precise pathogen identification for targeted treatment. The traditional culture methods used for diagnosing these infections have inherent limitations when it comes to detecting the diverse microbial flora present in HIV/AIDS patients.

Methods

In this study, sputum samples were collected from 37 HIV/AIDS patients admitted to Mengchao Hepatobiliary Hospital of Fujian Medical University. A total of 45 samples were subjected to analysis using three different methods: traditional culture methods, third-generation long-read sequencing (PacBio), and droplet digital PCR (ddPCR). The study aimed to compare the diagnostic capabilities and accuracy of microorganism identifi

SUBMITTER: Lai Y 

PROVIDER: S-EPMC12465504 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature