The Glycolysis-HIF-1α axis induces IL-1β of macrophages in rheumatoid arthritis.

ABSTRACT:

Background

Rheumatoid arthritis (RA) is an aggressive, systemic autoimmune disease in which overactivated macrophages play a critical role in its pathogenesis. This study aimed to explore the potential role of glycolytic reprogramming in the production of proinflammatory cytokines by macrophages in RA.

Methods

The Seahorse assay was conducted on RA or healthy control (HC) serum-treated human monocyte-derived macrophages (HMDMs) to evaluate glycolysis levels. RNA sequencing was performed to identify activated signaling pathways and key molecules in HMDMs stimulated by RA serum. The proinflammatory cytokines and hypoxia-inducible factor 1α (HIF-1α) were verified by Western blotting and quantitative polymerase chain reaction (qPCR).

Results

We found that HMDMs stimulated

SUBMITTER: Jia Y 

PROVIDER: S-EPMC12465665 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature