Genomic analyses of Purpureocillium lilacinum reveal unique DNA regions for developing isolate-specific molecular markers.

ABSTRACT:

Background

The Purpureocillium lilacinum NCHU-NPUST-175 (Pl-NCHU-NPUST-175) strain has been previously reported as an entomopathogenic fungus (EPF) with potential for controlling Forcipomyia taiwana (little black mosquito). In this study, genome sequencing of Pl-NCHU-NPUST-175 was performed via long-read sequencing (Oxford Nanopore Technologies, ONT). The comparative genomic analysis was conducted to identify isolate- and species-specific DNA regions for developing markers for rapid molecular identification, in order to detect the fungus in an environment and to confirm species identity before practical field applications.

Results

The genome assembly of Pl-NCHU-NPUST-175 is 36.55 Mb, consisting of 13 contigs with a GC content of 58.64%. The largest contig of the Pl-NCHU-NPUS

SUBMITTER: Yeh ZY 

PROVIDER: S-EPMC12465793 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature