A novel single-vector featuring bidirectional promoters for production of bispecific antibody against rabies virus.

ABSTRACT:

Background

The World Health Organization (WHO) recommends combination therapy with two non-overlapping monoclonal antibodies (mAbs) to enhance protection in Rabies post-exposure prophylaxis (PEP). While bispecific antibodies (bsAbs) provide superior protection over monoclonal antibodies (mAbs) by simultaneously targeting two distinct epitopes, their development is hindered by complex and inefficient vector design. In this study, we aimed to develop a novel single-vector expression system to efficiently produce potent anti-rabies virus (RABV) neutralizing bsAbs.

Results

We engineered a minimalistic peaSKYA single-vector featuring bidirectional dual-CMV promoters to enable synchronous co-expression of antibody heavy (HC) and light chains (LC) within a single construct. By elim

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12465811 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature