Up-regulating methylation of CAP1 promoter can promote apoptosis and inhibit migration and invasion of lung adenocarcinoma cells.

ABSTRACT:

Background

Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer, which poses a significant threat to human health. Adenylate cyclase-associated protein 1 (CAP1) is an important protein closely linked to cancer initiation and progression.

Method

Target Gene fragments were amplified by PCR, and the products of 2 fragments were ligated to construct pdCas9-Dnmt3a-BSD plasmid. Stable cell lines with methylation of CAP1 promoter up-regulated were then established through transfection and screening. Cell proliferation was assessed using colony formation and proliferation assays, while apoptosis was assessed by flow cytometry. Wound healing, transwell migration, and invasion assays were conducted to evaluate cell migration and invasion. Western blot and

SUBMITTER: Li G 

PROVIDER: S-EPMC12465864 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature