Role of SAA1 in mediating renal fibrosis through TLR4-dependent NF-κB activation.

ABSTRACT:

Background

Renal fibrosis is a key and irreversible pathological event in the progression of chronic kidney disease (CKD), and inflammatory response plays an important role in this process. Serum amyloid A1 (SAA1), an acute-phase reactant protein, which plays an important role in inflammation as a cytokine; but its specific role in renal fibrosis remains to be studied.

Methods

In this study, SAA1 and TLR4 knockout mice were used to establish unilateral ureteral obstruction (UUO) animal renal fibrosis model, and HK-2 cells were used to establish TGF-β-induced cell fibrosis model. The specific role of SAA1 in renal fibrosis was explored by immunoblotting and immunohistochemistry. Subsequently, this study used molecular docking and Co-Immunoprecipitation to verify the specific

SUBMITTER: Wei X 

PROVIDER: S-EPMC12465874 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature