Comprehensive multiomic analysis of extracellular vesicles from Mycoplasma bovis-infected bovine mammary epithelial cells identifies proteins and miRNAs that induce inflammatory responses in macrophages.

ABSTRACT: Mycoplasma bovis can lead to a decline in milk quality and yield, thereby causing significant economic losses worldwide. Extracellular vesicles (EVs) are crucial for triggering immune cell responses to infection. This study aimed to demonstrate the immunomodulatory effects of EVs released by bovine mammary epithelial cells (MAC-T cells) infected with M. bovis on bovine macrophages (BoMacs). After EVs were extracted from M. bovis-infected MAC-T cells (M. bovis NX2-EVs) as well as from uninfected MAC-T cells (Ctrl-EVs), they were incubated with BoMacs to assess their potential to induce cytokine expression. The results showed that M. bovis NX2-EV-treated BoMacs exhibited significantly increased expression of TNF-α, IL-1β, and IL-6. Additionally, the differentially expressed genes mainly invo

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12466063 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature