Immune checkpoint molecules and spatial transcriptome profiles according to BRAF status in acral melanoma.

ABSTRACT:

Background

Acral melanoma (AM) shows different genomic profiles based on BRAF status, and the data on the association with BRAF and immune checkpoint molecules are lacking.

Objectives

This study aimed to identify the significance of BRAF mutation in AMs, exploring expression patterns of immune checkpoint molecules and transcriptome profiles related to tumour immunity according to BRAF status.

Methods

Immunohistochemical (IHC) staining of BRAF, Programmed death-1 (PD-1), Lymphocyte-activation gene-3 (LAG-3) and T-Cell Immunoglobulin and mucin domain-3 (TIM-3) was performed on AM tissues. Through spatial transcriptome analysis, the correlation between BRAF expression and immune checkpoint molecule expression was examined. Analysis on differentially expressed genes along

SUBMITTER: Yang HJ 

PROVIDER: S-EPMC12466088 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature