Screening of polymorphic loci in candidate genes for colobomus eyelid and their application in phenotypic purification of geese.

ABSTRACT: This study was to screen, verify and apply molecular markers of candidate genes (FREM1 and PTPRM) related to eyelid defect, which may facilitate establishment of autosexing strains of Huoyan goose. Using 150 1-d-old Huoyan geese containing 100 with colobomus eyelid and 50 with normal eyelid, 6 and 3 polymorphic loci single nucleotide polymorphisms (SNPs) were identified in the 1-kb upstream sequences of FREM1 transcript 1 and transcript 2, respectively. All identified SNPs showed significant association with the colobomus eyelid trait (P < 0.05). To further investigate the regulatory effects of these SNPs, dual-luciferase reporter assays in goose primary hepatocytes and myocytes confirmed that mutations at SNP2 locus (c.-385 A > T) in transcript 1 and SNP3 locus (c.-714 A > G) in transcrip

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12466213 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature