Growth conditions shape the proteome and diversity of <i>Neurospora crassa</i> extracellular vesicles.

ABSTRACT: Extracellular vesicles (EVs) are nanosized, lipid bilayer-enclosed particles secreted by all living organisms. While EV research has primarily focused on mammalian systems, fungal EVs are gaining attention for their biological significance. Here, we investigated how growth conditions influence the protein cargo of EVs produced by Neurospora crassa, a non-pathogenic filamentous fungus and well-established model organism. EVs were isolated from cultures grown on glucose for 16 h (G16) and on sucrose for 16 (S16) and 24 h (S24). Dynamic light scattering (DLS) revealed similar size distributions for S16 and S24 EVs (24-165 nm), whereas G16 EVs exhibited a broader range (32-825 nm). Across all conditions, particles <50 nm were detected, potentially corresponding to mitochondrial-derived

SUBMITTER: Salgado-Bautista DA 

PROVIDER: S-EPMC12466246 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature