Phage Resistance Modulates <i>Escherichia coli</i> B Response to Metal-Based Antimicrobials.

ABSTRACT: Background/Objective: The rise of multidrug-resistant bacteria underscores the urgent need for alternative antimicrobial strategies. Metal-based compounds and bacteriophage (phage) therapy have emerged as promising candidates, but the evolutionary trade-offs associated with these selective pressures and their combination remain poorly understood. This study aimed to investigate how prior exposure to T4 phage influences Escherichia coli B's subsequent adaptation to iron (III) stress and to assess the resulting phenotypic and genomic signatures of dual resistance. Method: In this study, we performed experimental evolution using Escherichia coli B to investigate adaptive responses under four conditions: control (LB broth), T4 phage-only, iron (III) sulfate-only, an

SUBMITTER: Ezeanowai FC 

PROVIDER: S-EPMC12466345 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature