Multielemental Profile for Seminal Plasma Through Inductively Coupled Plasma-Tandem Mass Spectrometry and Its Relationship with Seminal Parameters, Spermatic Biomarkers, and Oxidative Stress.

ABSTRACT: The present study investigated the decline in human fertility by analyzing the multielemental profile of seminal plasma and its relationship with seminal parameters and sperm biomarkers. Twenty-nine donor seminal plasma samples were examined using inductively coupled plasma-tandem mass spectrometry (ICP-MS/MS). Method optimization demonstrated that robust plasma conditions, including internal standardization and helium (He) collision gas, were essential to achieve reliable quantification. These conditions mitigated matrix effects and spectroscopic interferences, despite lower sensitivity. Elements such as copper (Cu), iron (Fe), manganese (Mn), strontium (Sr), titanium (Ti), vanadium (V), and chromium (Cr) were quantified, and several significant correlations were identified. Specifically,

SUBMITTER: Lopez-Botella A 

PROVIDER: S-EPMC12466358 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature