A New Family-Based Approach for Detecting Allele-Specific Expression and for Mapping Possible eQTLs.

ABSTRACT: Allele-specific expression (ASE) reflects the unequal expression of the parental alleles and can imply functional variants in cis-regulatory elements. The conventional ASE detection methods often depend on the presence of heterozygous variants in transcripts or sequencing a large number of individuals, both of which are often limited. In this study, we present a family-based strategy for detecting ASE and potential cis-regulatory elements utilizing both RNA-seq and whole-genome sequencing (WGS) from a pedigree. Using a rabbit family consisting of two divergent parents and their eight offspring, we identified 913 ASE genes by analyzing inheritance patterns of gene expression levels. Expression was classified into three levels-high, medium, and low-and used to define seven distinct expressio

SUBMITTER: Alnajjar M 

PROVIDER: S-EPMC12466419 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature