Rapid Nanopore Sequencing to Identify Bacteria Causing Prosthetic Joint Infections.

ABSTRACT: Background/Objectives: The diagnosis of prosthetic joint infection remains difficult. Microbiological cultures frequently have false-positive and false-negative results. This study investigates whether rapid nanopore sequencing can be used to aid the identification of bacteria causing prosthetic joint infection for more timely identification and treatment. Methods: Nineteen patients who had revision surgery following total joint arthroplasty were included in this study. Of these, 15 patients had an infected joint arthroplasty. All patients had joint fluid aspirated at the time of revision surgery. The DNA was extracted from these fluid aspirates, and rapid nanopore sequencing was performed using the MinION device from Oxford Nanopore Technologies. The sequencing data was trim

SUBMITTER: Wilkinson H 

PROVIDER: S-EPMC12466696 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature