Temporizin-1 Meets the Membranes: Probing Membrane Inser-Tion and Disruption Mechanisms.

ABSTRACT: Background/Objectives: Temporizin-1, a hybrid antimicrobial peptide derived from the combination of Temporin A, Gramicidin peptide, and a poly-leu sequence, has strong trypanocide activity against Trypanosoma cruzi and moderate cytotoxicity towards mammalian cells. In this study, we investigated the mode of action of the peptide upon interaction with protozoan and eukaryotic membranes. Methods: To this end, we conducted a series of biophysical assays using liposomes as biomimetic models, along with fluorescence-based experiments such as lipid mixing, membrane leakage, and assays involving Thioflavin and Laurdan. Results: Temporizin-1 displayed potent membranolytic activity on protozoan and eukaryotic membranes, causing significant membrane fusion and leakage wit

SUBMITTER: Bellavita R 

PROVIDER: S-EPMC12466833 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature