Antifungal Susceptibility of <i>Malassezia pachydermatis</i> Isolates from Companion Animals and Genomic Insights into Resistance Mechanisms.

ABSTRACT: Background/Objectives: Malassezia pachydermatis is a lipophilic yeast frequently associated with otitis externa and dermatological disorders in companion animals. This study aimed to evaluate the antifungal susceptibility of M. pachydermatis isolates from dogs and cats and to investigate the genomic determinants of reduced susceptibility. Methods: Susceptibility testing of 87 clinical isolates was performed using a modified CLSI broth microdilution method in Sabouraud dextrose broth supplemented with 1% Tween 80. The whole genome of ten representative isolates was sequenced and the genetic factors that are involved in drug resistance were investigated. Results: Ketoconazole, itraconazole, and terbinafine exhibited the highest efficacy, while miconazole an

SUBMITTER: Doman M 

PROVIDER: S-EPMC12466836 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature