Supercomplex Restructuring in Heart Mitochondria of COX7A1-Deficient Mice.

ABSTRACT: The role of electron transport chain supercomplexes and factors that regulate their composition in a tissue- and species-specific manner are not fully understood. Tissue-specific isoforms have been reported for cytochrome c oxidase (COX), which may contribute to such regulation. Therefore, we here investigated COX activity and structural organization in wild-type (WT) and COX7A1 knockout (KO) mice, which lack the heart/skeletal muscle isoform of COX subunit VIIa. COX7A1 KO mice showed a 30% reduction in total COX activity in the heart. Although the activity of COX in the monomers and I+III₂+IV_n supercomplexes (SCs) remained unchanged, a marked reduction in COX dimers and unknown COX-containing species IV_x and IV_y contributed to the overall

SUBMITTER: Pavelich L 

PROVIDER: S-EPMC12467167 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature