Whole-Cell Bioreporter-Based Assay for Detecting Fungal-Derived β-Lactamase Inhibitors.

ABSTRACT: β-lactams are an important family of antibiotics that are prone to undergo resistance inhibition though the production of β-lactamases by some microorganisms. To combat this resistance and preserve the efficacy of β-lactam antibiotics, we developed a strategy for the discovery of such β-lactamase inhibitors. When combined with β-lactams, these inhibitors allow the antibiotics to be effective and prevent resistance. To date, the development of such combinatory drugs is limited due to the complexity of screening for new β-lactamase inhibitors. Therefore, to facilitate this development, it was essential to find sensitive assays to effectively screen for lactamase inhibitory compounds. To this end, a novel bioassay utilizing bioluminescent indicator bacteria as bioreporters was developed. The

SUBMITTER: Benou R 

PROVIDER: S-EPMC12467308 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature