Proof-of-Concept Study: Hyperspectral Imaging for Quantification of DKK-3 Expression in Oropharyngeal Carcinoma.

ABSTRACT:

Introduction

Oral squamous cell carcinoma (OSCC) is one of the most common tumours worldwide. This study investigated the suitability of visible and near-infrared hyperspectral imaging compared to visual assessment and conventional digital image analysis for quantifying immunohistochemical staining on the example of Dickkopf-3 (DKK-3) in OSCC.

Materials and methods

A retrospective analysis of TMAs containing DKK-3 stained OSCC of 50 patients was retrieved from the archives at the Institute of Pathology, Medical University of Innsbruck. TMAs were first evaluated visually, followed by digital image analysis using QuPath (version 0.3.2, open-source software). For hyperspectral imaging, six exemplary cases were selected (three cases with strong expression and three cases with we

SUBMITTER: Mittermair T 

PROVIDER: S-EPMC12467325 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature