An Isothermal Deoxyribozyme Sensor for Rapid Detection of Enteroviral RNA.

ABSTRACT: Enteric viruses are a major cause of waterborne infections due to their high environmental stability and extremely low infectious dose. Current molecular diagnostic methods, while accurate, often depend on thermal cycling and centralized laboratory facilities, limiting their applicability in decentralized or resource-limited settings. In this study, we developed an isothermal biosensor based on a split deoxyribozyme that reconstitutes its catalytic core upon hybridization with a conserved sequence of enteroviral RNA. This activation leads to site-specific cleavage of a fluorogenic substrate, producing a quantifiable fluorescent signal. The system was experimentally validated using both synthetic enteroviral RNA and RNA extracted from environmental water samples. To enhance detection sensit

SUBMITTER: Sas B 

PROVIDER: S-EPMC12467329 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature