Evaluation of Hypovirus Infection on the Vesicular Protein Expression Pattern of <i>Cryphonectria parasitica</i> by TMT-Based Proteomics Analysis.

ABSTRACT: Hypovirus infection is known to reduce the pathogenicity of Cryphonectria parasitica, the causative agent of chestnut blight. Isoforms derived from a viral protein p48 have been discovered in host mitochondria and vesicles, which may contribute to virulence attenuation, as reported in earlier work using two-dimensional electrophoresis (2-DE). In this study, a total of 1739 fungal proteins were identified in fungal vesicles through Tandem Mass Tag (TMT)-based quantitative proteomics. The infection of CHV1-EP713 was associated with 75 up-regulated and 201 down-regulated proteins, predominantly involved in vesicular transport process and related cellular functions, including protein folding, membrane fusion, retrograde transport, autophagy, and ER stress responses. The down-regulation

SUBMITTER: Zeng Z 

PROVIDER: S-EPMC12467672 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature