Characterization of Two Novel Heat Shock Protein 70 Transcripts from <i>Sitodiplosis mosellana</i> and Their Response to Larval Diapause and Thermal Stress.

ABSTRACT: The heat shock protein 70 (Hsp70) family mediates responses to environmental stress in insects. The wheat midge Sitodiplosis mosellana, a worldwide pest, avoids summer and winter temperature extremes by diapause of the third-instar larvae in the soil. To explore the functions of Hsp70s in this process, we characterized two cytoplasmic Hsp70 genes (SmHsp70A1-1 and SmHsp70A1-2) from this insect. Both SmHsp70s contained three signature motifs of the family and lacked introns. Developmental expression profiling revealed maximal SmHsp70A1-1 expression during early larval stages, while the expression of SmHsp70A1-2 was highest in the pupal stages. The expression of SmHsp70A1-1 was significantly upregulated during diapause, particularly during summer and

SUBMITTER: Huang Q 

PROVIDER: S-EPMC12467714 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature