CD45 and Basigin (CD147) Are Functional Ligands for Galectin-8 on Human Leukocytes.

ABSTRACT: The interactions of leukocyte glycoproteins with adhesion and signaling molecules through glycan recognition are not well understood. We previously demonstrated that galectin-8, a tandem-repeat lectin with N- and C-terminal carbohydrate binding domains which is highly expressed in endothelial and epithelial cells, can bind to activated neutrophils to induce surface exposure of phosphatidylserine (PS) without DNA fragmentation or apoptosis, in a process termed preaparesis. However, the receptors for Gal-8 on leukocytes have not been identified. Here we report our results using both proteomics and affinity chromatography with both full-length Gal-8 and the separate Gal-8 C-terminal and N-terminal domains to identify glycoprotein ligands in HL-60 cells for Gal-8. Two of the major ligan

SUBMITTER: Gourdine JF 

PROVIDER: S-EPMC12467718 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature