A Liposomal Formulation Enhances the Anti-Senescence Properties of Nicotinamide Adenine-Dinucleotide (NAD<sup>+</sup>) in Endothelial Cells and Keratinocytes.
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ABSTRACT: Nicotinamide adenine-dinucleotide (NAD+) supplementation is a promising strategy to delay cellular aging in different areas, including cosmetic dermatology. However, low bioavailability and stability of NAD+ formulations are the main factors limiting its effectiveness as an anti-aging treatment. In light of the above, a liposomal formulation of NAD+ (LF-NAD+) was tested in this study and compared to NAD+ alone in primary human aortic endothelial cells (HAECs) and primary human epidermal keratinocytes (HEKas). Intracellular NAD+ was measured using a colorimetric assay. Cell survival was derived from lactate dehydrogenase release in supernatants. Cell senescence was measured by senescence-associated β-galactosidase staining. M
SUBMITTER: Ministrini S
PROVIDER: S-EPMC12468054 | biostudies-literature | 2025 Sep
REPOSITORIES: biostudies-literature
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