Fecal Metabolomics for the Diagnosis of <i>Clostridioides difficile</i> Infection.

ABSTRACT: Background:Clostridioides difficile infection (CDI) is the leading cause of nosocomial diarrhea. Current diagnostic tools have difficulty distinguishing between colonization and active infection. This study evaluated the utility of fecal metabolomics in diagnosing CDI in hospitalized patients with acute diarrhea. Methods: We conducted a prospective observational study involving hospitalized adults with new-onset diarrhea during admission. Participants were stratified into groups based on clinical and microbiological findings: controls, C. difficile colonized and C. difficile infected. Fecal samples were analyzed using UPLC-MS/MS and GC-MS to quantify selected short-chain fatty acids, amino acids, and bile acids. Multivariate and univariate statistical ana

SUBMITTER: Bea-Serrano C 

PROVIDER: S-EPMC12468941 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature