Characterizing oxidative metabolites of 6-methylnicotine (6MN; aka Metatine): divergent metabolism from nicotine and identification of urinary biomarkers of exposure.

ABSTRACT: The emergence of synthetic nicotine analogs in "tobacco-free" products, such as 6-methylnicotine (6MN; aka Metatine) in SPREE BAR, presents new regulatory and public health challenges. Alarmingly, little is known about the metabolism of 6MN, its potential biomarkers of exposure, or its toxicity. In this study, we systematically characterized oxidized metabolites of 6MN in the urine of mice exposed to 6MN (via intraperitoneal or inhalation route) using liquid chromatography-high resolution mass spectrometry. Similarly, human urine samples were analyzed for 6MN metabolites after use of the SPREE BAR (Blue Razz Ice) product. Nine 6MN metabolites were identified in mouse urine, and each metabolite corresponded with a known nicotine metabolite, albeit with increased mass (i.e. m/z + 14 Da). Alt

SUBMITTER: Xie Z 

PROVIDER: S-EPMC12469198 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature