PAFAH1B3 Exists in Linear Chromosomal and Extrachromosomal Circular DNA and Promotes HCC Progression via EMT.

ABSTRACT: Recent evidence highlights the role of extrachromosomal circular DNAs (eccDNAs) in cancers. However, reports regarding its role in hepatocellular carcinoma (HCC) are infrequent. The abundance of eccDNAs from five HCC/adjacent tissue pairs was explored using Circle-Sequencing. eccDNA PAFAH1B3 was selected as one of the objects. The effect of eccDNA PAFAH1B3 on HCC progression was determined using EdU, Transwell, and apoptosis assays. Additionally, the expressions of eccDNA PAFAH1B3, mRNA PAFAH1B3, and epithelial-mesenchymal transition (EMT)-related markers were determined using RT-PCR and WB. A xenograft tumor model was established to explore the function of PAFAH1B3 in vivo, and EMT-related markers were detected using RT-PCR and IHC analyses. The abundance of eccDNA PAFAH1B3 was significan

SUBMITTER: Li D 

PROVIDER: S-EPMC12469353 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature