MicroRNAs Secreted by the Embryo in Spent Culture Medium Can Regulate mRNAs Involved in Endometrial Receptivity, Embryo Attachment, and Invasion.

ABSTRACT: MicroRNAs, secreted by the embryo in blastocoel fluid (BF) and embryo spent culture medium (SCM), regulate important cellular pathways controlling the stemness of inner cell mass, trophectoderm differentiation, and the dialogue between blastocyst and maternal tissues. In recent years, their role as non-invasive biomarkers of embryo quality has been deeply investigated. We compared the expression profiles of 96 microRNAs between BF and SCM from the same embryos, highlighting the differences between these two compartments. We found 10 and 6 microRNAs specifically expressed in BF and in SCM, respectively; 22 microRNAs significantly up-regulated in BF; and 2 significantly up-regulated in SCM. To investigate the role of SCM microRNAs in implantation, we focused on the microRNAs specifically exp

SUBMITTER: Caponnetto A 

PROVIDER: S-EPMC12469383 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature