Rules of Engagement for Components of Membrane Protein Biogenesis at the Human Endoplasmic Reticulum.

ABSTRACT: In human cells, the biogenesis of membrane proteins, which account for one quarter of polypeptides and sixty percent of human drug targets, is initiated at the membrane of the endoplasmic reticulum (ER). This process involves N-terminal signal peptides or transmembrane helices in the membrane protein precursors. Over one hundred proteins enable membrane-targeting and -insertion of the precursors as well as their folding and covalent modifications. Four targeting pathways to the Sec61 channel in the ER membrane with their effectors and three cooperating or independent membrane protein-insertases have been identified. We combined knock-down of individual components of these pathways and insertases in HeLa cells with label-free quantitative mass spectrometric analysis of the proteomes. Differ

SUBMITTER: Zimmermann R 

PROVIDER: S-EPMC12469465 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature