Protective Mechanism of Broad Bean Extract on Parkinson's Disease Model Cells.

ABSTRACT: Broad beans, natural sources of L-DOPA and bioactive phenolics show promise for Parkinson's disease intervention. This study investigated broad bean extracts' protective mechanisms against PD pathogenesis. Among screened varieties, QC25 extract exhibited optimal protection in MPP⁺-injured PC12 cells, improving viability, reducing LDH release, and mitigating cell cycle arrest. QC25 extract rescued mitochondrial dysfunction by suppressing ROS, restoring membrane potential, normalizing Ca²⁺ homeostasis, and recovering ATP synthesis. Metabolomics identified glycerophospholipid metabolism as the core protective pathway, mediating mitochondrial membrane stabilization. QC25 extract further activated PINK1/Parkin-mediated mitophagy, upregulating PINK1 and Parkin expression. C

SUBMITTER: Chen X 

PROVIDER: S-EPMC12469533 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature