The Omega-3 Fatty Acids Eicosapentaenoic Acid and Docosahexaenoic Acid Enhance the Effects of Temozolomide Chemotherapy in Glioblastoma Cells.

ABSTRACT: Overcoming resistance to temozolomide (TMZ) chemotherapy is a major challenge in glioma treatment. Polyunsaturated fatty acids (PUFAs) can interfere with drug resistance in glioma but their mechanism of action is poorly understood. Eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA) effects were assessed using proliferation and clonogenic assays, adhesion and migration assays, glucose and lactate metabolism, detection of lipid droplets, acidic vesicles, reactive oxygen species, and phosphokinase proteome analysis. EPA or DHA caused significant reductions in cell number, colony formation, adhesion, and migration in comparison with TMZ alone, increasing lipid droplet accumulation, reactive oxygen species formation, acidic vesicle number and apoptosis, while also altering glucose and la

SUBMITTER: Silva JA 

PROVIDER: S-EPMC12469626 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature