Integrating Transcriptomics, Network Pharmacology, and Machine Learning to Reveal Transglutaminase 2 (TGM2) as a Key Target Mediating Taurocholate Efficacy in Colitis.

ABSTRACT:

Background

Ulcerative colitis (UC) is a chronic inflammatory disease of the colon with a rising global incidence. Natural conjugated taurocholic acid (TCA) possesses anti-inflammatory properties and shows potential therapeutic effects against UC, although the underlying mechanisms remain unclear.

Methods

This study employed an integrative approach-combining network pharmacology, bioinformatics, machine learning, immune infiltration analysis, and molecular docking-to investigate the therapeutic mechanisms of TCA in UC. UC-related gene expression datasets were obtained from the Gene Expression Omnibus (GEO) database, and potential TCA targets were predicted using the Comparative Toxicogenomics Database (CTD) and TargetNet platforms. Differentially expressed genes (DEGs) were i

SUBMITTER: Zhu J 

PROVIDER: S-EPMC12469685 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature