Sequential Reconstruction of Calicheamicin γ₁ ^I Iodo-Thiobenzoate by Selective Carrier Protein Trapping Reveals a Flavin-Dependent Iodinase.

ABSTRACT: Flavin-dependent halogenases (FDHs) play important roles in natural product biosynthesis, particularly chlorinases and brominases. Apart from ubiquitous mammalian iodination in thyroxine biosynthesis, iodinated natural products are extremely rare, and enzymes responsible for iodination are even less well described. A notable exception is calicheamicin γ₁ ^I, a potent antitumor compound containing an aromatic iodide, for which a halogenase has been proposed to mediate iodine incorporation. Despite predictions regarding the enzymes involved in iodinated aryl ring biosynthesis, experimental evidence remains limited due to challenges in substrate identification and reaction monitoring. In this study, we successfully reconstituted the enzymatic activities required for iodin

SUBMITTER: Pang F 

PROVIDER: S-EPMC12469858 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature