Insights into the Metabolic Adaptations of a Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Strain on Exposure to Sublethal Concentrations of Ertapenem.

ABSTRACT: Klebsiella pneumoniae strains that are resistant to carbapenems are of great concern. Exposure to low concentrations of antibiotics may influence tolerance to antibiotics. Novel antibiotics and treatment options are thus needed, and this need is exacerbated by the rapid and global spread of antibiotic resistance. In this study, we determined the global proteome changes in a K. pneumoniae strain (CCUG 70747) carrying carbapenem resistance genes when exposed to low concentrations of ertapenem. Quantitative proteomics was achieved by the tandem mass tag labeling of peptides generated by trypsin proteolysis and mass spectrometry analysis. Bioinformatics analyses were used to observe changes in protein abundance, as well as the gene ontology (GO) terms and pathways associated with

SUBMITTER: Jaen-Luchoro D 

PROVIDER: S-EPMC12470040 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature