Mixture Deconvolution with Massively Parallel Sequencing Data: Microhaplotypes Versus Short Tandem Repeats.

ABSTRACT: Background/Objectives: Interpretation of mixture profiles generated from crime scene samples is an important element in forensic genetics. Here, a workflow for mixture deconvolution of sequenced microhaplotypes (MHs) and STRs using the probabilistic genotyping software MPSproto v0.9.7 was developed, and the performance of the two types of loci was compared. Methods: Sequencing data from a custom panel of 74 MHs (the MH-74 plex) and a commercial kit with 26 autosomal STRs (the ForenSeq™ DNA Signature Prep Kit) were used. Single-source profiles were computationally combined to create 360 two-person and 336 three-person mixtures using the Python script MixtureSimulator v1.0. Additionally, 72 real mixtures typed with the MH-74 plex and 18 real mixtures typed with the ForenSeq Kit

SUBMITTER: Giuffrida M 

PROVIDER: S-EPMC12470170 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature