Haplotype-Phased Chromosome-Level Genome Assembly of <i>Floccularia luteovirens</i> Provides Insights into Its Taxonomy, Adaptive Evolution, and Biosynthetic Potential.

ABSTRACT: Floccularia luteovirens is a valuable medicinal and edible ectomycorrhizal fungus that is endemic to alpine meadows on the Qinghai-Tibet Plateau. It is of significant ecological and pharmacological importance. To overcome the genomic limitations of previous fragmented assemblies, we present the first haplotype-phased, chromosome-scale genome of the Qinghai-derived QHU-1 strain using an integrated approach of PacBio HiFi, Hi-C, and Illumina sequencing. The high-contiguity assembly spans 13 chromosomes with 97.6% BUSCO completeness. Phylogenomic analysis of 31 basidiomycetes clarified a historical misclassification by placing F. luteovirens closest to Mycocalia denudata/Crucibulum laeve, thus confirming its distinct lineage from Armillaria spp. through low synteny

SUBMITTER: Qi J 

PROVIDER: S-EPMC12470553 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature