Analysis of Non-Polar Low-Molecular Metabolites in Citron (<i>Citrus medica</i> L.) Peel Essential Oil at Different Developmental Stages and a Combined Study of Transcriptomics Revealed Genes Related to the Synthesis Regulation of the Monoterpenoid Compound Nerol.

ABSTRACT: Citron (Citrus medica L.) peel essential oil is rich in terpenoids, but their composition and formation mechanisms remain unclear. We analyzed volatile compounds and gene expression in citron peel across three developmental stages using GC-MS and RNA-seq. GC-MS identified 91 volatiles, with γ-Terpinene and D-Limonene the most abundant; terpenes dominated (95.60-87.72%) but decreased with maturation, with 19 differential terpenoid metabolites. Differential expression analysis identified 10,238 genes with significant changes across three stages, including 256 TPS genes and 656 transcription factors. Via WGCNA and correlation analysis, 10 gene-metabolite pairs were identified, including 5 key genes and 2 transcription factors linked to nerol synthesis. These findings provide a framewor

SUBMITTER: Luo J 

PROVIDER: S-EPMC12470865 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature