Transcriptomic Analysis of Osmotic Stress-Tolerant Somatic Embryos of <i>Coffea arabica</i> L. Mediated by the Coffee Antisense <i>Trehalase</i> Gene: A Marker-Free Approach.

ABSTRACT: Coffee Coffea arabica L. depends on abundantly distributed rainfall, and drought negatively impacts plant development, fruit production, bean quality, and, ultimately, beverage quality. Plant biotechnology by means of genetic manipulation and plant regeneration by the somatic embryogenic process is an alternative technology to overcome these problems. In the present work, we used the molecular approach of the Trehalase gene silencing to allow trehalose accumulation favoring plants surviving in extreme drought/salt environments. We used a cassette containing the antisense C. arabica L. Trehalase gene under the RD29 promoter from A. thaliana and the NOS terminator to genetically modify an embryogenic coffee C. arabica L. cv Typica line under osmotic

SUBMITTER: Valencia-Lozano E 

PROVIDER: S-EPMC12471068 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature