Comparative Proteomic Analysis of Extracellular Vesicles from Donkey Colostrum and Mature Milk.

ABSTRACT: Background/Objectives: Donkey milk (DM) has been considered a valuable alternative to human and bovine counterparts as well as to infant formulas. Milk extracellular vesicles (EVs) have been proposed to influence key biological processes. The purpose of this study is to provide a comprehensive characterization of the protein composition of extracellular vesicles (EVs) by extending quantitative proteomic comparisons to EVs derived from donkey colostrum (DC) and mature donkey milk (MDM). Methods: The EVs were isolated from DC and MDM samples, characterized, and subjected to proteomic analysis using the tandem mass tag-based quantitative approach. Results: In addition to typical milk proteins and EV markers, EVs from DC and MDM both contain components associated with the

SUBMITTER: Caira S 

PROVIDER: S-EPMC12472028 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature